Evaluation Of Effect Of Amalaki Churna (Emblica Officinalis Gaertn.) As Rasayana And Its Free Radicals Scavenging Activity In Healthy Individuals

Abstract

Objectives: To screen comparative study of Amalaki churna (Emblica officinalis Gaertn.) as Rasayana in three different Oushadha Sevan Kala as Kinchit Suryodayajate (Sunrise), Divas bhojane (Midday meal) and Nishi (Night meal), and Regular food is given to Control group. Methodology: 1. A total of 100 healthy individual were selected and divided into 4 groups. a) Group 1; Amalaki churna was given for 30 days during (Kinchit Suryodayajate) during sunrise process time (06:00 a.m.) b) Group 2: Amalaki churna was given for 30 days at Midday meal time (12:00 pm) c) Group 3: Amalaki churna was given for 30 days at Night time (08:00 p.m.) d) Control group 4: Regular food was given for 30 days e) Strict time schedule was maintained throughout the procedure. Blood samples (nearly about 10 ml of each healthy individual) of all 100 subjects were taken in unbreakable non- vacuum blood collection tubes before starting the study and collected blood samples were transferred to centrifuge machine for centrifugation. After 5 minutes of centrifugation serum was collected at top (approximately about 5 ml) of the tubes, then serum was preserved in deep freezer at -800 C at Lyka Labs Limited, Manufacturers of Pharmaceuticals, 4801/B, 4802/A, G.I.D.C. Industrial Estate, Ankleshwar-393002, Dist. Bharuch, Gujarat, India. Then from next day administration of Amalaki churna (Emblica officinalis Gaertn.) was started for period of 30 days. After completion of 30 days again blood samples were collected in unbreakable non-vaccume tubes transferred it to centrifuge machine, collect the serum. Next to that readings were taken. 2. Rasayana study carried out as per Superoxide dismutase (SOD) and Catalase (CAT) on Healthy individuals using Amalaki Churna at dose of 3 grams O.D. respectively and findings were analyzed by using one way ANOVA, tukeys multiple posthoc procedure and dependent ‘t’ test Page III Abstract Results: 1) The serum SOD levels were significantly decreased in group 1 (8.09 units/ml.) as compared to control (14.37 units/ml.), group 2 (9.74 units/ml.) and group 3 (9.77 units/ml.) respectively. Conclusions: Evaluation of Oushadha Sevan Kala i.e. time of administration of medicine was the main objective of the study which comes back with highly significant results, on the basis of Oushadha Sevan Kala. Amalaki boost weakened antioxidant defenses in Kinchit suryodayajate kala (morning time of administration) Medicine given at the appropriate time will be conducive for the better treatment. The Bheshaja Sevana Kala (time of administration of medicine) is mainly governed by dominance of particular dosha which is responsible for biological rhythms and is targeted for the treatment. The drug optimization can be achieved by administering the medicine in an appropriate time. For management of Rasayana Karma the ideal Bheshaja Sevana Kala is Kinchita suryodayajate as per clinical and experimental evidence. Bheshaja Sevana Kala is having its own scope and application in the management of diseases. By incorporating proper Bheshaja Sevana Kala one can enhance the bioavailability, target the disease site and relieve symptoms.