Abstract:
First order derivative spectroscopy, Dual Wavelength Spectroscopy and RP-HPLC methods were developed and validated for simultaneous estimation of Olopatadine Hydrochloride and Ambroxol Hydrochloride in bulk and It’s Dosage form. Simple and easy UV spectrophotometric methods with good sensitivity have been developed for simultaneous quantification of Olopatadine Hydrochloride and Ambroxol Hydrochloride. The method employed First order derivative method based on the measurement of absorbance at two wavelengths, 302.6 and 248.34 nm, ZCP of Olopatadine Hydrochloride and Ambroxol Hydrochloride, respectively. The calibration curve was linear in a concentration range of 5-15 μg/ml for Olopatadine Hydrochloride and 30-90 μg/ml for Ambroxol Hydrochloride. The % recoveries of the both the drugs were found to be 99.37- 100.53 % and 98.95– 100.11%. The Dual Wavelength Spectroscopy Method based on measurement of the absorbance difference between two wavelengths, for Dual Wavelength method linearity was obtained in the concentration range 5-15μg/ml for Olopatadine Hydrochloride and 30-90 μg/ml for Ambroxol Hydrochloride at absorbance difference between 242.9nm and First order derivative spectroscopy, Dual Wavelength Spectroscopy and RP-HPLC methods were developed and validated for simultaneous estimation of Olopatadine Hydrochloride and Ambroxol Hydrochloride in bulk and It’s Dosage form. Simple and easy UV spectrophotometric methods with good sensitivity have been developed for simultaneous quantification of Olopatadine Hydrochloride and Ambroxol Hydrochloride. The method employed First order derivative method based on the measurement of absorbance at two wavelengths, 302.6 and 248.34 nm, ZCP of Olopatadine Hydrochloride and Ambroxol Hydrochloride, respectively. The calibration curve was linear in a concentration range of 5-15 μg/ml for Olopatadine Hydrochloride and 30-90 μg/ml for Ambroxol Hydrochloride. The % recoveries of the both the drugs were found to be 99.37- 100.53 % and 98.95– 100.11%. The Dual Wavelength Spectroscopy Method based on measurement of the absorbance difference between two wavelengths, for Dual Wavelength method linearity was obtained in the concentration range 5-15μg/ml for Olopatadine Hydrochloride and 30-90 μg/ml for Ambroxol Hydrochloride at absorbance difference between 242.9nm and
