Abstract:
Present work describes a precise, accurate and reproducible absorbance ratio, first order derivative spectroscopy and Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) methods for simultaneous estimation of Lornoxicam and Eperisone. The absorbance ratio (Q-analysis) method was based on the measurement of absorbances at two wavelengths, one being the iso-absorptive point at 291.5 nm (λ1) and other being λmax, 254.5 nm (λ2) of one of the sample components. The second method was based on the use of first order derivative spectroscopy, in which derivative amplitudes were measured at selected wavelengths 254 nm (ZCP of EPE) for LXM and 264.5 nm (ZCP of LXM) for EPE in 0.1N methanolic NaoH, without mutual interference. Linearity was found in the concentration range of 0.16 – 0.96μg mL and 2 - 12μg/mL, respectively for Lornoxicam and Eperisone in API mixture for both methods. In the RP-HPLC method, the drugs were resolved in a synthetic mixture, using Enable C18H column (250 mm x 4.6 mm, i.d. 5μm particle Present work describes a precise, accurate and reproducible absorbance ratio, first order derivative spectroscopy and Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) methods for simultaneous estimation of Lornoxicam and Eperisone. The absorbance ratio (Q-analysis) method was based on the measurement of absorbances at two wavelengths, one being the iso-absorptive point at 291.5 nm (λ1) and other being λmax, 254.5 nm (λ2) of one of the sample components. The second method was based on the use of first order derivative spectroscopy, in which derivative amplitudes were measured at selected wavelengths 254 nm (ZCP of EPE) for LXM and 264.5 nm (ZCP of LXM) for EPE in 0.1N methanolic NaoH, without mutual interference. Linearity was found in the concentration range of 0.16 – 0.96μg mL and 2 - 12μg/mL, respectively for Lornoxicam and Eperisone in API mixture for both methods. In the RP-HPLC method, the drugs were resolved in a synthetic mixture, using Enable C18H column (250 mm x 4.6 mm, i.d. 5μm particle
