Abstract:
A rapid and sensitive liquid chromatography-tandem mass spectroscopy (LC-MS/MS)
method for the determination of Linezolid in human plasma using Linezolid D8 as
Internal Standard has been developed & validated, the analytical method consist of
Solid Phase Extraction by using Orochem Cartridges. Samples were than analysed by
HPLC on a column, Chromolith (5 μm, 100×4.6mm) using mobile phase consisting
of Acetonitrile:5mM Ammonium Formate (85:15) delivered at 0.8mL/min with 85%
of splitting. Detection was performed using an Applied Biosystem MDS SCIEX API
3000 mass spectrometer. Electron Spray Ionization was used for ion production. The
assays were linear over the range 100 –24000 ng/mL with intra & inter day precision
of 0.28 to 7.39%, accuracy in the range of 95.6 to 102.6% & mean recovery was
101.09%. The intended analyte is stable at below 10°C in all the preformed
experiments and the stability experiments preformed are within the acceptance limits.
This method can be used for quantification of Linezolid in human plasma for
Bioequivalence studies.
