Evaluation of the effect of the Solanum nigrum fruits in experimentally induced Acute pancreatitis in rats

Abstract

Objectives: Evaluation of the effect of the Solanum nigrum fruits in experimentally induced Acute pancreatitis in rats. Material and methods: In this model of Pancreatitis, 36 wistar rats of either sex were divided in to 6 groups. Group-1 served as a Normal control which received 0.5% CMC and other group will receive L-arginine (2 × 250mg/100 gm body weight, intraperitoneal, 1 h apart). Group-2 served as a Disease control (DC). Where as a group-3 served as a standard group (STD) which received Prednisolone 30mg/kg and group-4, 5, and 6 were a treatment groups that received methanolic extract of Solanum nigrum with the dose of 200 mg/kg, 400 mg/kg and 600 mg/kg respectively. After the experimental period blood sample were collected for serum parameter (Amylase, Lipase, Nitric oxide and C-reactive protein). Moreover, Pancreas was isolated for biochemical parameters (Superoxide dismutase, Lipid peroxidase and Myeloperoxidase) and histopathological studies. Results: L-arginine treatment caused Pancreatitis as evidenced by marked elevation in Blood amylase, lipase. Co-administration of herbal extract decreased rise in Blood amylase & lipase level. Apart from these Histopathological changes also showed curative nature of herbal extract against L-arginine induced pancreatic damage. Conclusion: It was observed that herbal extract of Solanum nigrum fruits has the potential to combat acute pancreatitis by acting as an anti-inflammatory and antioxidant agent by histopathological, serum and biochemical observation against L-arginine induced pancreatitis in rats.