Evaluation of the effect of the abutilon indicum linn. In experimentally induced acute pancreatitis in rats

Abstract

Objectives: Evaluation of the effect of the Abutilon indicum Linn. in experimentally induced Acute pancreatitis in rats. Material and methods: Thirty Wistar rats of either sex were divided into five groups with 6 rats each: Group-1 served as a Normal control (NC) which receive vehicle (0.5% CMC) and other group will receive L-arginine (2 × 250 mg/100 gm body weight, intraperitoneal, 1 h apart). Group-2 served as a Disease control (DC). Where as a group-3 served as a standard group (STD) which receive Prednisolone 30 mg/kg and group-4 and 5 served as a treatment group that receive methanolic extract of A.indicum which were treated with the dose of 250 mg/kg (AI 250) and 500 mg/kg (AI 500) respectively. After the experimental period blood sample were collected for serum parameter (Amylase, Lipase, Nitric oxide and C-reactive protein). Moreover, Pancreas was isolated for biochemical parameters (Superoxide dismutase, Lipid peroxidase and Myeloperoxidase) and histopathological studies were obtained. Results: L-arginine treatment caused Pancreatitis as evidenced by marked elevation in Blood amylase, lipase. Co-administration of methanolic extract of A.indicum decreased rise in Blood amylase & lipase level. Estimation of biochemical parameters like MDA, MPO shows decreases and increase in level of SOD after administration of methanolic extract of A.indicum. Apart from these Histopathological changes also showed curative nature of methanolic extract of A.indicum against L-arginine induced pancreatic damage. Conclusion: It was observed that methanolic extract of A.indicum whole plant has the potential to combat acute pancreatitis by acting as an anti-inflammatory and antioxidant agent by histopathological, serum and biochemical observation against L-arginine induced pancreatitis in rats.