Abstract:
The aim of the present investigation was to prepare and evaluate of nanoparticles
based formulation of silver sulphadiazine for treatment of burn wound and targeting
towards the bacterial infection and mucoadhesion can be achieved by using
hyaluronic acid. Silver sulphadiazine (SSD) can improve the infectious condition if
given in the form of nanoparticles based formulation. Fourier transform infrared
spectroscopy (FTIR) had employed to study drug-excipients incompatibility.
Optimization of formulation parameter was done by Box Behnken Design (BBD)
using Design Expert software. Nanoparticles based formulation of silver
sulphadiazine was evaluated for particle size, percent drug entrapment, zeta potential,
in-vitro drug release study surface morphology, antimicrobial study, skin irritation
study, in vivo wound healing study and stability study. FTIR study shows that neither
drug decomposition nor drug-excipients and excipient-excipient interactions occurred
in the formulation. Nanoparticles based formulation of silver sulphadiazine was
successfully prepared using chitosan, hyaluronic acid and sodium tripolyphosphate.
Particle size, zeta potential and percent drug entrapment were found to be 371.1 nm,
+24.6 mV and 92.53±0.92 % respectively for optimized batch. Transmission electron
microscopy study indicates that the particles were found to be in spherical shape. Invitro
drug release of optimized batch was found to be 90.72±0.46 % up to 12 hrs.
Antimicrobial study shows that silver sulphadiazine posses most promising
antimicrobial activity and it is as effective as silver sulphadiazine marketed
formulation. Skin irritation study shows that formulation was not irritated to the skin. In vivo wound healing study shows that nanoparticles based formulation posses good
wound healing activity as marketed formulation. Stability study indicates that
formulation containing silver sulphadiazine nanoparticles was stable at 4-8 ± 2°C/45
±5% RH (Refrigerator; RF) and 25 ± 2°C/ 65 ± 5% RH (Room temperature; RT). The
present study demonstrated that, nanoparticles based formulation for treatment of burn
wound hold great potential for treating bacterial infection in burn wound.
