Abstract:
Problem statement Helicobacter pylori are one of the most common infectious
agents, gram negative bacteria which are responsible for gastric ulcer and gastric
adenocarcinoma. According to WHO and International Agency for Research, H.
Pylori is a type I carcinogen. H. Pylori is adapted to live in the acidic environment of
the stomach but it change their environment and allowing them to survive. The
objective of this study was to prepare metronidazole containing floating in-situ gel
which was resolved the problem created by h. pylori. In systemic drug delivery high
dose required and some time not enough dose at the site, not got a proper result. To
overcome this problem, site targeted formulation which was prepared minimum dose
and gives sustain release of drug. By using hot method metronidazole containing insitu
gel was prepared using Gellan gum as an polymer. Gellan gum was selected after
the comparision between HPMC and Na-CMC. Gellan gum containing in-situ gel
gives higher drug content and it is required for site targeted formulation. A1 to A10
batches were taken for optimization of polymer and floating agent concentration. In
that A6 batch optimized based on viscosity, gelling capacity and %CDR. It gives
sustained drug release till 9 hours. After applying 23 full factorial design, 9 batches
were taken and from that F7 batch was optimized based on viscosity, % drug content
and % drug release. Metronidazole containing floating in-situ gel was successfully
prepared by hot method.
