DETERMINATION OF AFLATOXIN M1 IN MILK AND DEGRADATION THROUGH BIFIDOBACTERIUM INFANTIS STRAIN

Abstract

Milk and milk items are a significant commitment to the human eating routine particularly kids. Be that as it may, the nearness of aflatoxins as AFM1 in milk and milk items are viewed as nuisances because of their wellbeing dangers in buyer's body. Hence, this investigation intended to survey the capacity of some microbial species on aflatoxin evacuation particularly the AFM1 in the milk.The blend of nonviable probiotic bacterial and yeast species (Lactobacillus acidophilus, Bifidobacterium Infantis) prevailing to diminish AFM1 from 50 (ng/ml) during the hatching time frames; 12 h, 24 h, 48 h and 72 h, into 9.72±1.31, 6.68±0.55, 5.70±0.33 and 4.56±0.15 ng ml-1, individually. The most noteworthy AFM1 expulsion % was recorded as; 80.56%, 86.64%, 88.60% and 90.88% in the treated milk tests in an individual way.The compelling chromatographic detachment and quantitation of aflatoxin M1 utilizing a PerkinElmer Altus HPLC framework with an A-10 FL locator and immunoaffinity SPE test cleanup. The outcomes showed remarkable linearity over the tried fixation go. Quantitation is reachable down to < 0.02 ppb, with very great recuperations. The sub-0.02 ppb LOQ takes into account the normal examination of aflatoxin M1, well beneath the 0.05 ppb satisfactory breaking point in milk, as set up by the EU.