http://localhost:8080/xmlui/handle/123456789/7300 2026-04-05T17:41:49Z http://localhost:8080/xmlui/handle/123456789/7318 Development and validation of analytical methods for Simultaneous estimation of atorvastatin calcium and Irbesartan in their pharmaceutical dosage form. SHAH, SHRADDHA A simple, accurate, and precise UV spectroscopy and RP-HPLC methods were developed and validated for simultaneous estimation of Atorvastatin Calcium and Irbesartan in their Pharmaceutical Dosage Form. A simple and sensitive UV spectrophotometric method has been developed by Simultaneous equation method in which determination was carried out at 246nm λmax of Atorvastatin Calcium and 228nm λmax of Irbesartan. The calibration curves were linear in a concentration range of 6-26 μg/ml for Atorvastatin Calcium and 8-18 μg/ml for Irbesartan at their respective wavelength. In the combined Dosage Form both the drugs were estimated as 99.20% and 99.66% Atorvastatin Calcium and Irbesartan respectively. The RPHPLC method was performed on ProntoSIL C18 column (150mm X 4.6 mm i.d., 5 μm particle size) with a gradient system of Acetonitrile : Buffer (pH 3.5) in the ratio of 55:45 v/v with a flow rate of 1.0 ml/min, injection volume 20μl and detection wavelength was 245nm. The retention time for Atorvastatin Calcium and Irbesartan was obtained as 5.930±0.05min and 2.92±0.1min respectively. The linearity of the proposed method was investigated in the range of 2-10μg/ml and 15-75μg/ml for Atorvastatin Calcium and Irbesartan respectively. Correlation coefficient was 0.9992 and 0.9994 for Atorvastatin Calcium and Irbesartan respectively. The developed method was validated as per ICH guidelines, for its accuracy, precision, LOD & LOQ and satisfactory results were obtained, thus the method is specific, rapid, and sensitive for estimation of Atorvastatin Calcium and Irbesartan. For Full Thesis Kindly contact to respective Library 2015-04-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7316 Stability Indicating Analytical method Devlopment and Validation for Simultaneous Estimation of Paracetamol and Zaltoprofen in Solid Dosage Dadhania, Ruchi Objective: Stability Indicating Analytical method Devlopment and Validation for Simultaneous Estimation of Paracetamol and Zaltoprofen in Solid Dosage Form. Experimental work done: UV spectrophotometric estimation was carried out by First order derivative method. Methanol was used as a solvent for UV spectrophotometric method. HPLC method was performed by using C18, 5μ, 250 mm x 4.6 mm. Various trials were performed for optimization of mobile phase. All these methods were validated as per ICH guidelines.Various Stability parameters were also checked for the degradation of drugs. Results and Discussion: The selected optimized wavelength for spectrophotometric methods, in First order derivative method were 268 nm and 248 nm, for estimation of ZALTO, and PCM respectively. The optimized mobile phase for HPLC method phosphate buffer : Acetonitrile pH 3.0 with OPA in the ratio of 65: 35 v/v gave peak of ZALTO and PCM at Retention Time of 4.83 min. and 2.79 min. at λmax=260 nm. % RSD for precision, accuracy and robustness for all methods was less than 2. In Stability study HCl, NaOH, H2O2 were used in acid, Base and Oxidation and Thermal Degradation respectively. Conclusion: The spectrophotometric and chromatographic methods developed are sensitive, precise, accurate and reproducible for analysis of pharmaceutical formulation. Results of method suggest that all the methods are repeatable and specific for the estimation of ZALTO and PCM. Stability study suggest that no any interference observed from any degradant peaks at retention time of ZALTO and PCM. For Full Thesis Kindly contact to respective Library 2015-04-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7315 Development and validation of stability indicating Assay method for simultaneous estimation of Furosemide and triamterene in tablet dosage form Joshi, Maulik The present work involves the development and validation of a simple, accurate and precise U.V spectrophotometric method, RP-HPLC and Forced Degradation Study Method for the assay of Triamterene and Furosemide. Simple, specific, accurate, precise and reproducible method have been developed and validated for the simultaneous estimation of both drugs in their combined dosage form. UVspectrophotometric method was a determination using the derivative spectrophotometry at 268 nm and 328 nm over the concentration range 2-12 μg/ml and 4-14 μg/ml for Triamterene and Furosemide in methanol respectively. The % recoveries of the both the drugs were found to be 99.56-101.07% and 99.11-100.85% respectively. The Stability Indicating RP-HPLC method has shown adequate separation of Triamterene and Furosemide in its tablet dosage Form. The separation was achieved on a Enable C18 H(250mm X 4.6 mm i.d., 5 μm particle size) with a isocratic system of 0.1% Formic Acid in Water : Acetonitrile(65:35%v/v) the mobile phase at a flow rate of 0.8 ml/min, Injection volume 10μl and wavelength of detection used was 274nm. The retention time for Triamterene and Furosemide was obtained as 1.948±0.1min and3.753 ±0.1min, respectively. The linearity of the proposed method was investigated in the range of 8-12 μg/ml and 6.4-9.6 μg/ml and coefficient was 0.999 and 0.994 for Triamterene and Furosemide, respectively. The developed method was validated as per ICH guideline and the results were found to be satisfactory, thus the method is specific, rapid and simple with good sensitivity for estimation of Triamterene and Furosemide For Full Thesis Kindly contact to respective Library 2015-04-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/7313 Analytical method development and validation for Simultaneous estimation of pravastatin sodium and Fenofibrate in combination SAVALIA, KHUSHBU A First order derivative spectroscopic method, Absorbance Correction spectroscopic method and RP-HPLC method were developed and validated for simultaneous estimation of Pravastatin sodium and Fenofibrate in combination. A simple and rapid UV spectrophotometric methods has been developed for simultaneous quantification of Pravastatin sodium and Fenofibrate. First order derivative method based on the measurement of absorbance at two wavelengths, 275 nm and 239 nm, ZCP of Pravastatin sodium and Fenofibrate respectively. The calibration curve was linear in a concentration range of 1-6 μg/ml for Pravastatin sodium and 4-24 μg/ml for Fenofibrate. Absorbance Correction method based on the measurement of absorbance at two wavelengths, 237 nm for Pravastatin sodium and 286 nm for Fenofibrate. The calibration graph was linear in the concentration range of 1-6 μg/ml for Pravastatin sodium and 4-24 μg/ml for Fenofibrate. The RP-HPLC method performed on a Phenomenex Luna C18 (250mm X 4.6 mm i.d., 5 μm particle size) with an Isocratic system of Methanol : Buffer (pH 3) in the ratio of 90:10 v/v at flow rate of 1.0 ml/min and wavelength of detection used was 247 nm. The retention time for Pravastatin sodium and Fenofibrate was obtained at 3.13 min and 7.32 min, respectively. Calibration curves were linear (R2 = 0.998 for Pravastatin Sodium and 0.998 for Fenofibrate) in the concentration range of 2–12 and 8–48 μg/ml for Pravastatin sodium and Fenofibrate, respectively. The developed method was validated as per ICH guideline. For Full Thesis Kindly contact to respective Library 2015-04-01T00:00:00Z