http://localhost:8080/xmlui/handle/123456789/10552 2026-04-12T22:49:49Z http://localhost:8080/xmlui/handle/123456789/10560 QUALITATIVE AND QUANTITATIVE ANALYSIS OF ACHYRANTHES ASPERA MAKWANA, MEGHA KANUBHAI Achyranthes aspera is an important medicinal herb found as a weed throughout India. The presence of a various phytochemicals was discovered by the qualitative analysis of Achyranthes aspera. During this study, different extracts (methanol, ethyl acetate, aqueous, chloroform) of leaves and stem of Achyranthes aspera was used. Phytochemicals such as, Alkaloids, Carbohydrates, Flavonoids, Steroids, Glycosides, Phenols, Protein & Amino acids, reducing sugars, Coumarin, Tannins and Terpenoids were screened from the leaves and stem of Achyranthes aspera. Methanol extract was screened for estimation of total flavonoid and total phenol content. In the present study, qualitative analysis of stem and leaf shows absence and presence of these phytochemicals and quantitative analysis of flavonoid and phenol shows the concentration of it. For Full Thesis Kindly contact to respective Library 2020-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10559 STUDIES ON QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF MORINDA CITRIFOLIA PANJROLIA, SHRUTI GAJENDRASINH Morinda citrifolia has been reported to have a broad range of therapeutic and nutritional values. For further investigation, the qualitative and quantitative phytochemical studies were carried out on leaf, fruits, and stem plant parts of Morinda citrifolia. This plant has been used in treating various diseases such as, heart disease, cancer, hypertension, arthritis, diabetes, muscle pain, etc. Qualitative chemical analysis was done using different extracts (methanol, ethyl acetate, aqueous and chloroform) and the studies indicate the presence of various secondary metabolites such as alkaloids, flavonoids, terpenoids, steroids, reducing sugars, glycosides, and tannins. Quantitative chemical analysis was also performed to determine the total flavonoids and phenol contents. Quantitative analysis showed highest amount of flavonoids content (2.31 mg QE/gm) and total phenol content (1.405 mg GAE/gm) in fruit in methanolic extract. For Full Thesis Kindly contact to respective Library 2020-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10558 Phytochemical Analysis & Antimicrobial Activity of Peristrophe Bicalyculata WADHWANI, KAJOL LAXMANKUMAR Peristrophe Bicalyculata have been reported to have many insecticidal and anti-bacterial properties. For further investigation, the phytochemical, anti-microbial and anti-fungal studies were carried out on the leaf part of the plant. This plant has been used in treating various skin diseases. It is very useful in the treatment of T.B, Jaundice, Antiseptic and Anti-venom agent in the traditional system of medicine. The screening indicates the presence of various secondary metabolites such as tannins, alkaloids, flavonoids, glycosides, terpenoids, reducing sugars and saponins. Ethanolic and aqueous extracts were tested against five different microorganisms. Anti-fungal studies were carried out against two fungi named Aspergillus Niger and Candida albicans. Anti-microbial as well as anti-bacterial studies were carried out against Bacillus subtilis, Escherichia coli and Staphylococcus Aureus. Anti-microbial activity was carried out at 100mg/ml concentration by Agar Well Diffusion method. The evaluation revealed the ethanolic extract was most effective against S.Aureus and Bacillus subtilis. No extract was active against Aspergillus Niger. Ethanolic extracts were most effective in bacteria than fungi. From the evaluation, the ethanolic extracts of Peristrophe Bicalyculata posses a good source of various new drugs for treating various pathogens and infections. For Full Thesis Kindly contact to respective Library 2020-01-01T00:00:00Z http://localhost:8080/xmlui/handle/123456789/10557 DETERMINATION OF AFLATOXIN M1 IN MILK AND DEGRADATION THROUGH BIFIDOBACTERIUM INFANTIS STRAIN TANDAN, GIRIDHAR DEVDAS Milk and milk items are a significant commitment to the human eating routine particularly kids. Be that as it may, the nearness of aflatoxins as AFM1 in milk and milk items are viewed as nuisances because of their wellbeing dangers in buyer's body. Hence, this investigation intended to survey the capacity of some microbial species on aflatoxin evacuation particularly the AFM1 in the milk.The blend of nonviable probiotic bacterial and yeast species (Lactobacillus acidophilus, Bifidobacterium Infantis) prevailing to diminish AFM1 from 50 (ng/ml) during the hatching time frames; 12 h, 24 h, 48 h and 72 h, into 9.72±1.31, 6.68±0.55, 5.70±0.33 and 4.56±0.15 ng ml-1, individually. The most noteworthy AFM1 expulsion % was recorded as; 80.56%, 86.64%, 88.60% and 90.88% in the treated milk tests in an individual way.The compelling chromatographic detachment and quantitation of aflatoxin M1 utilizing a PerkinElmer Altus HPLC framework with an A-10 FL locator and immunoaffinity SPE test cleanup. The outcomes showed remarkable linearity over the tried fixation go. Quantitation is reachable down to < 0.02 ppb, with very great recuperations. The sub-0.02 ppb LOQ takes into account the normal examination of aflatoxin M1, well beneath the 0.05 ppb satisfactory breaking point in milk, as set up by the EU. For Full Thesis Kindly contact to respective Library 2020-01-01T00:00:00Z